Human umbilical cord blood derived mesenchymal cells for the treatment of myocardial infarction in rat model

Abstract

Objectives: The present study was designed to know the possible protective and regenerative effects of human Umbilical Cord Blood (UCB) derived MSCs in Myocardia Infarction (MI) in rat model using Isoproterenol administration.

Material & methods: Isoproterenol injection was given subcutaneously for 2 days to induce MI in rat model. After development of MI various blood and tissue parameters were assessed sich as urea, creatinine, SGOT, SGPT, troponin I, Lactic Dehydrogenase (LDH) and Creatine Kinase (CK). Treatment was given by single dose of MSCs. After treatment period all the parameters were assessed again and comparison were done before and after treatment.

Results: After development of MI all the biochemical parameters were elevated. MSC transplantation, performed when MI have already developed, showed better results. It inhibits the degradation of normal collagen and the formation of poorly cross-linked collagens, resulting in attenuation of MI and improvement of heart function by means of troponin I, Lactic Dehydrogenase (LDH) and Creatine Kinase (CK) level. In addition, this impact is not instantaneous but is persistent for at least 90 days, supporting the role of MSC transplantation.

Conclusion: Our data suggested that transplantation of MSCs in MI model has improved the cardiac function. Till now, there is no evidence attributing this improvement to regeneration and other paracrine mechanisms are also believed to contribute in the improvement of cardiac function.